Quantitative Metabolomics& Lipogenomics Platform

Untargeted Lipidomics System

Basic Information
Vendor:Thermo Fisher Scientific
Instrument:Exploris 480
Reservation Call:(021) 31246790
Location:A101 Life Sciences Building

Performance & Specification

  • Resolution

    480,000 at m/z 200, 8 levels option

  • Mass range

    m/z 40–6,000

  • Scan rate

    Up to 40 Hz at resolution setting 7,500 at m/z 200

  • Mass accuracy

    < 3 ppm with external calibration

    < 1 ppm using internal standard, lock masses

  • Sensitivity

    MS/MS: 100 fg reserpine on column S/N 150:1

    SIM: 50 fg reserpine on column S/N 150:1

  • Polarity switching

    One full experimental cycle acquired in at >1.4 Hz where the cycle consists of acquiring one full scan MS in positive and negative polarity at a resolution setting of 60,000

  • Multiplexing

    Up to 20 precursors per scan

Application

  • Proteomics: Protein identification, Post-translational modification, Biomacromolecule Interactions, Quantitative analysis of peptides and proteins.

  • New Drug R&D, Drug Impurity identification,Metabolism Identification, Metabolomics, Lipidomics, Natural product, Multi-omics, Interaction of small molecules and biological macromolecules.

Capillary Electrophoresis

Basic Information
Vendor:AB SCIEX
Instrument:CESI8000+
Reservation Call :  (021)31246790
Location:Room A101, Building of Life Sciences

Instrument Specification

  1. Capillary temperature control: liquid medium, temperature range 15-60℃, Accuracy 0.1℃.

  2. Sample temperature control: semiconductor, temperature range 4-60℃, Accuracy 0.1℃.

  3. Buffer rack: 2×36 =72 for 1.8 mL vial.

  4. Sample rack: 2×48 =96 for 1.8 mL vial and PCR tube.

Untargeted Metabolomics System

Basic Information
Vendor:AB SCIEX
Instrument:TripleTOF6600+
Reservation Call:(021)31246790
Location: Room A101, Building of Life Sciences

Instrument Specification

  1. Mass range(m/z): 5-2250 amu for quadrupole,5-40,000 amu for TOF.

  2. Resolution: ≥50000(FWHM),at m/z 956.

  3. Accuracy (MS and MS/MS): <2.0 ppm(external calibration).Scan speed: 200 Hz.

  4. SWATH acquisition mode: Sequential Windowed Acquisition of All Theoretical Spectra for Comprehensive Qualification and Quantification.

  5. Scan mode: MS, MS/MS, NL, MS-IDA-MS/MS, DBS.

Targeted Lipidomics System

Basic Information
Vendor:AB SCIEX
Instrument:QTRAP5500+
Reservation Call :  (021)31246790
Location :  Room A101, Building of Life Sciences

Instrument Specification

  1. Instrument: Triple quadrupole and linear ion trap hybrid mass spectrometry operating in MS, MS/MS, and MS/MS/MS scan modes for quantification and qualification.

  2. Mass range (m/z): 5-1250 amu.

  3. Polarity Switch Time: ≤ 5ms without losing sensitivity and reproducibility.

  4. Scan speed: ≥ 20000 Da/sec.

  5. Quantification: linearity≥106, Reproducibility <5% (RSD).

  6. Scan mode: Full Scan, SIM, SRM, Production Ion Scan, Precursor Ion Scan, Neutral Loss Scan, MRM, Mix Scan Mode, IDA, EMS, EMC, EPI, ER, TDF, MS/MS/MS, MRM3.

Targeted Metabolomics System

Basic Information
Vendor:AB SCIEX
Instrument:QTRAP6500+
Reservation Call :  (021)31246790
Location:Room A101, Building of Life Sciences

Instrument Specification

  1. Instrument: Triple quadrupole and linear ion trap hybrid mass spectrometry operating in MS, MS/MS, and MS/MS/MS scan modes for quantification and qualification.

  2. Mass range (m/z): : 5-2000 amu.

  3. Polarity Switch Time: ≤ 5 ms without losing sensitivity and reproducibility.

  4. Scan speed: ≥ 20000 Da/sec.

  5. Quantification: linearity≥106, Reproducibility <5% (RSD).

  6. Scan mode: Full Scan, SIM, SRM, Production Ion Scan, Precursor Ion Scan, Neutral Loss Scan, MRM, Mix Scan Mode, IDA, EMS, EMC, EPI, ER, TDF, MS/MS/MS, MRM3.