Cell Biological Imaging Platform

Multidimensional Image Analysis Workstation

Basic Information
Instrument:Bitplane Imaris 9.3.1
Reservation Call: (021)31246791
Location: Room A101-3, Building of Life Sciences

Instrument Specification

  1. Reconstruct and Visualize complex 3/4D microscopy datasets up to terabytes in size.

  2. Precisely detect and segment objects using the wizard-driven segmentation tools in Imaris.

  3. Calculate size and shape parameters, signal intensity, and relative position in space for all detected objects.

  4. Track objects over time (4D tracking) using the highly reliable algorithms, and display motion related parameters: speed, displacement vectors, track straightness, cell divisions, lineage tree, etc.

  5. Detect, trace and measure neurons’ dendritic trees, axons, spines, blood vessels and microtubules.

  6. Detect cells based on cytoplasm labeling or based on plasma membrane labeling, and examine relationships between cells and cellular components.

  7. Check colocalization of fluorescent signal from various components in 3D over time.

  8. GPU-accelerated Deconvolution to improve image quality.

High-content Screening System

Basic Information
Instrument: Opera Phenix
Reservation Call: (021)31246791
Location :  Room A101-3, Building of Life Sciences

Instrument Specification

  1. Spinning-disk optics and careful synchronization of laser excitation and camera exposure minimize bleaching and phototoxicity.

  2. Proprietary automated water-immersion objectives with very high numerical aperture deliver and capture more photons and provide higher resolution in XYZ than conventional air objectives.

  3. Up to two large-format sCMOS cameras deliver low noise, wide dynamic range, and high resolution – perfect for sensitive and quantitative measurements at short exposure times.

  4. The Opera Phenix system's Synchrony Optics feature a microlens-enhanced Nipkow disk with increased transmission rates for fast, sensitive, true-multipoint confocal imaging. The system's unique dual-view layout separates the excitation and emission paths for fluorophores with overlapping spectra by creating non-overlapping pinhole patterns in the sample. This minimizes crosstalk during simultaneous confocal acquisition by 98% on average, so you can simultaneously acquire images of the nucleus (labeled with Hoechst) and of the cytoplasm (labeled with GFP, for example) and up to two more markers – without limiting sensitivity.

Super Resolution Multimode Microscopic Imaging System for Living Cells

Basic Information
Vendor: NIKON
Instrument: A1&SIM&STORM
Reservation Call: (021)31246791
Location :  Room A101-5, Building of Life Sciences

Instrument Specification

  1. Combined with laser scanning confocal (Confocal), structured illumination microscopy (SIM) and stochastic optical reconstruction microscopy (STORM) technology.

  2. The spatial resolution of SIM is up to 20 nm, which can realize multi-channel 3D fluorescence imaging.

  3. High-speed piezoelectric 3D scanning electric stage, 32-channel spectral imaging, perfect focus system (PFS) are suitable for long-term observation of living cells.

  4. Suitable for immune calibration of fixed cells and fluorescent living cells.

Function Application

Cell migration and cytoskeleton; cell division and cell cycle; cell signal transduction; tissue differentiation and development; vesicles and protein transport; physiology and neuroscience; calcium signal research; protein-DNA interaction; Host - pathogen interaction; Cancer research; Pharmacology research; Biophysics research. Research on living microorganisms, proteins, viruses, subcellular structures, etc.

Ultra-Fast and High Resolution Laser Confocal Microscope System

Basic Information
Instrument:LSM 880 with Airyscan
Reservation Call: (021)31246791
Location : Room A101-4, Building of Life Sciences

Instrument Specification

  1. Airyscan detector for 2x increase in spatial resolution in each dimension (120 nm lateral and 350 nm axial resolution), 4-8 increase in SNR.

  2. 19 fps @ 512x512 at Fast Airyscan mode.

  3. Spectra imaging and Linear unmixing with a 32 channels spectral detector for 6-9 color fluorescent imaging.

  4. Advance confocal function, such as FRAP, FRET, TILE, Multi-dimension with Experiment Designer Module.

Function Application

  • Cell Biology Imaging

  • Molecular Localization

  • Pharmacokinetics