Basic Information
Vendor：PE
Instrument： Opera Phenix
Reservation Call： (021)31246791
Location :  Room A101-3, Building of Life Sciences

Instrument Specification

1. Spinning-disk optics and careful synchronization of laser excitation and camera exposure minimize bleaching and phototoxicity.

2. Proprietary automated water-immersion objectives with very high numerical aperture deliver and capture more photons and provide higher resolution in XYZ than conventional air objectives.

3. Up to two large-format sCMOS cameras deliver low noise, wide dynamic range, and high resolution – perfect for sensitive and quantitative measurements at short exposure times.

4. The Opera Phenix system's Synchrony Optics feature a microlens-enhanced Nipkow disk with increased transmission rates for fast, sensitive, true-multipoint confocal imaging. The system's unique dual-view layout separates the excitation and emission paths for fluorophores with overlapping spectra by creating non-overlapping pinhole patterns in the sample. This minimizes crosstalk during simultaneous confocal acquisition by 98% on average, so you can simultaneously acquire images of the nucleus (labeled with Hoechst) and of the cytoplasm (labeled with GFP, for example) and up to two more markers – without limiting sensitivity.