青年研究员、国家优青获得者

电话：021-31242082

邮箱：wanglingbo@fudan.edu.cn

办公地址：复旦大学2号交叉学科楼D7009室

个人简介

汪凌波博士，课题组长、博士生导师。2017年7月于中国科学院生物化学与细胞生物学研究所(中科院分子细胞科学卓越创新中心)获得博士学位，师承李劲松院士；2017年7月至2020年3月于复旦大学从事博士后研究；2020年6月加入复旦大学附属妇产科医院，PI、博导；2022年7月加入复旦大学代谢与整合生物学研究院，PI、博导。近五年，主持多个国家级和省部级科研项目。

主要研究方向

“疾病动物建模及干预”课题组致力于重大人类遗传病的小鼠建模与机制研究。运用基因编辑、胚胎显微操作、胚胎干细胞等相关技术方法，以基因编辑小鼠模型和疾病临床家系相结合，探索基因突变导致疾病的遗传机制及其干预方法。课题组主要研究兴趣包括：（1）开发基因修饰小鼠建模技术；（2）建立人源化基因编辑小鼠模型（包括不孕不育等疾病模型），阐明发病机制；（3）利用小鼠模型，开展疾病的干预方法研究。

常用技术：小鼠胚胎显微操作技术、胚胎干细胞技术、CRISPR/Cas9、Base Editing等。

本课题组常年招收博士后、研究生、科研助理等人才，欢迎有志从事科学研究的青年才俊报考本课题组攻读博士、硕士学位。

荣誉及获奖情况

• 2023年 国家优青 

• 2019年 上海市青年科技启明星
  

代表性成果

*: corresponding；#: co-first

1. Wang L#, Li M#, Qu C, Miao W, Yin Q, Liao J, Cao H, Huang M, Wang K, Zuo E, Peng G, Zhang S, Chen G, Li Q, Tang K, Yu Q, Li Z, Wong C, Xu G, Jing N, Yu X*, Li J*. CRISPR-Cas9-mediated genome editing in one blastomere of two-cell embryos reveals a novel Tet3 function in regulating neocortical development. Cell Research, 2017, 27:815-829.

2. Wang L#, Zhang Y#, Fu X#, Dong S#, Tang S, Zhang N, Song C, Yang N, Zhang L, Wang H, Shi H, Jin L, Zhang F*, Li J*, Hua K*. Joint utilization of genetic analysis and semi-cloning technology reveals a digenic etiology of Müllerian anomalies. Cell Research, 2020, 30:91-94.

3. Wang Y#, Chen J#, Huang X#, Wu B, Dai P, Zhang F, Li J, Wang L*. Gene-knockout by iSTOP enables rapid reproductive disease modeling and phenotyping in germ cells of the founder generation. SCIENCE CHINA-Life Sciences, 2023, doi:10.1007/s11427-023-2408-2.  (*: corresponding author)

4. Cong J#, Yang Y#, Wang X#, Shen Y#, Qi H#, Liu C, Tang S, Wu S, Tian S, Zhou Y, He X, Wang L*, Liu M*, Zhang F*. Deficiency of X-linked TENT5D causes male infertility by disrupting the mRNA stability during spermatogenesis.Cell Discovery, 2022, 8: 23. (*:corresponding authors)

5. Wang Y#, Huang X#, Sun G, Chen J, Wu B, Luo J, Tang S, Dai P, Zhang F, Li J, Wang L*. Coiled-coil domain-containing 38 is required for acrosome biogenesis and fibrous sheath assembly in mice. Journal of Genetics and Genomics, 2023, doi: 10.1016/j.jgg.2023.09.002. https://doi.org/10.1016/j.jgg.2023.09.002. (*: corresponding author)

6. Chen J, Wang Y, Wu B, Shi H*, Wang L*. Experimental and molecular support for Cfap70 as a causative gene of Multiple Morphological Abnormalities of the Flagella with male infertility. Biology of Reproduction, 2023, 109(4):450-460, doi: 10.1093/biolre/ioad076. https://doi.org/10.1093/biolre/ioad076.  (*: corresponding author)

7. Meng G#, Wang Y#, Luo C#, Tan Y, Li Y, Tan C, Tu C, Zhang Q, Hu L, Zhang H, Meng L, Liu C, Deng L, Lu G, Lin G, Du J, Tan Y, Sha Y*, Wang L*, He W*. Bi-allelic variants in DNAH3 cause male infertility with asthenoteratozoospermia in humans and mice. Human Reproduction Open, 2024, hoae003, doi:10.1093/hropen/hoae003.  https://doi.org/10.1093/hropen/hoae003.  (*: corresponding authors)

8. Zhou Y#, Wang Y#, Chen J, Wu B, Tang S, Zhang F*, Liu C*, Wang L*. Dnali1 is required for sperm motility and male fertility in mice. Basic and Clinical Andrology, 2023, 33:32, doi:10.1186/s12610-023-00205-y.  (*: corresponding author)

9. Wang L, Li J. Expansion of the mutant monkey through cloning. SCIENCE CHINA-Life Sciences, 2019, 62, 865-867.

10. Wang L, Li J. ‘Artificial spermatid’-mediated genome editing. Biology of Reproduction, 2019, 101, 538-548.

11. Zhang X#, Wang L#, Ma Y#, Wang Y#, Liu H#, Liu M#, Qin L#, Li J, Jiang C, Zhang X, Shan X, Liu Y, Li J, Li Y, Zheng R, Sun Y, Sun J, Leng X, Liang Y, Zhang F*, Jiang X*, Yang Y*, Shen Y*. CEP128 is involved in spermatogenesis in humans and mice. Nature Communications, 2022, 13: 1395. (#: co-first authors)

12. Liu C#, Tu C#, Wang L#, Wu H#, Houston B, Mastrorosa F, Zhang W, Shen Y, Wang J, Shixiong Tian, Meng L, Cong J, Yang S, Jiang Y, Tang S, Zeng Y, Lv M, Lin G, Li J, Saiyin H, He X, Jin L, Touré A, Ray P, Veltman J, Shi Q, O’Bryan M, Cao Y, Tan Y*, Zhang F*.  Deleterious variants in X-linked CFAP47 induce asthenoteratozoospermia and primary male infertility. American Journal of Human Genetics, 2021, 108:309-323. (#: co-first authors)

13. Liu C#, He X#, Liu W#, Yang S#, Wang L#, Li W, Wu H, Tang S, Ni X, Wang J, Gao Y, Tian S, Zhang L, Cong J, Zhang Z, Tan Q, Zhang J, Li H, Zhong Y, Lv M, Li J, Jin L, Cao Y*, Zhang F*. Bi-allelic mutations in TTC29 cause male subfertility with asthenoteratospermia in humans and mice. American Journal of Human Genetics, 2019, 105:1168-1181. (#: co-first authors)

14. Li G#, Yang X#, Wang L#, Pan Y, Chen S, Shang L, Zhang Y, Wu Y, Zhou Z, Chen Q, Zhang X, Zhang L, Wang Y, Li J, Jin L, Wu Y*, Zhang X*, Zhang F*. Haploinsufficiency in non-homologous end joining factor 1 induces ovarian dysfunction in humans and mice. Journal of Medical Genetics, 2022, 59:579–588. (#: co-first authors)

15. Cong J#, Wang X#, Amiri-Yekta A#, Wang L#, Kherraf Z, Liu C, Cazin C, Tang S, Hosseini S, Tian S, Daneshipour A, Wang J, Zhou Y, Zeng Y, Yang S, He X, Li J, Cao Y,Jin L, Ray P*, Zhang F*. Homozygous mutations in CCDC34 cause male infertility with oligoasthenoteratozoospermia in humans and mice. Journal of Medical Genetics, 2022, 59:710–718. (#: co-first authors)

16. Dai P#, Wang X#, Gou L#, Li Z#, Wen Z#, Chen Z#, Hua M, Zhong A, Wang L, Su H, Wan H, Qian K, Liao L, Li J, Tian B, Li D, Fu X, Shi H*, Zhou Y*, Liu M*. A translation-activating function of MIWI/piRNA during mouse spermiogenesis. Cell, 2019, 179:1566-1581 e1516.

17. Ke H#, Tang S#, Guo T#, Hou D, Jiao X, Li S, Luo W, Xu B, Zhao S, Li G, Zhang X, Xu S, Wang L, Wu Y, Wang J, Zhang F*, Qin Y*, Jin L*, Chen Z*. 2023. Landscape of pathogenic mutations in premature ovarian insufficiency. Nature Medicine, 2023, 29(2): 483-492.

18. Jiang J#, Lv W#, Ye X, Wang L, Zhang M, Yang H, Okuka M, Zhou C, Zhang X, Liu L*, Li J*. Zscan4 promotes genomic stability during reprogramming and dramatically improves the quality of iPS cells as demonstrated by tetraploid complementation. Cell Research, 2013, 23, 92-106.

19. Liu P, Dou X, Liu C, Wang L, Xing C, Peng G, Chen J, Yu F, Qiao Y, Song L, Wu Y, Yue C, Li J, Han J, Tang K, Jing N. Histone deacetylation promotes mouse neural induction by restricting Nodal-dependent mesendoderm fate. Nature Communications, 2015, 6:6830.

20. Wang X#, Qiao Y#, Xiao M#, Wang L, Chen J, Lv W, Xu L, Li Y, Wang Y, Tan M, Huang C, Li J, Zhao T, Hou Z*, Jing N*, Chin Y*. Opposing roles of acetylation and phosphorylation in LIFR-dependent self-renewal growth signaling in mouse embryonic stem cells. Cell Reports, 2017, 18: 933-946.

21. Chen Q, Ke H, Luo X, Wang L, Wu Y, Tang S, Li J, Jin L, Zhang F*, Qin Y*, Chen X*. Rare deleterious BUB1B variants induce premature ovarian insufficiency and early menopause.Human Molecular Genetics, 2020, 29, 2698-2707.

22. Qu C#, Yan M#, Yang S, Wang L, Yin Q, Liu Y, Chen Y, Li J. Haploid embryonic stem cells can be enriched and maintained by simple filtration. Journal of Biological Chemistry, 2018, 293:5230-5235. 

23. Yang J, Guo R, Wang H, Ye X, Zhou Z, Dan J, Wang H, Gong P, Deng W, Yin Y, Mao S, Wang L, Ding J, Li J, Keefe D, Dawlaty M, Wang J, Xu G, Liu L. Tet enzymes regulate telomere maintenance and chromosomal stability of mouse ESCs. Cell Reports, 2016, 15, 1809-1821. 

24. Zhang J#, Tang S#*, Zhu X, Li P, Lu J, Cong J, Wang L, Zhang F, Li Z*. Asian Journal of Andrology, 23, 288–293.